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Please direct all comments, questions, and suggestions to biobzc@hofstra.edu
Protocols
The protocols below were written as instructions for the students. Notes to instructors can be found at the end of each protocol. Throughout these instructions I refer to the "common mutant" and an "unknown." These refer to the Mhc mutant and the student's assigned line for the independent project respectively.
General |
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| Agarose Gel Electrophoresis | |
| Measuring Optical Density | |
| Precipitation of DNA | |
| Use of Enzymes | |
| UV Spectrophotometry | |
Laboratory Specific |
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| Laboratory 1-2: | Characterization of Mutants | |
| Laboratory 2: | Plasmid Rescue Overview | |
| Laboratory 3: | Chromosomal DNA | |
| Laboratory 4: | Preparation of Competent Cells | |
| Laboratory 4: | Digestion and Ligation | |
| Laboratory 5: | Transformation | |
| Laboratory 6: | Plasmid DNA Preparation | |
| Laboratory 7-10: | RNA Preparation | |
| Laboratory 10-12: | PCR Analysis Splicing Variants | |
| Laboratory 8: | Preparation of DNA for Sequencing | |
| Laboratory 8-10: | Southern and Northern Analysis | |
| Laboratory 11-16: | Screening a cDNA Library | |
| Laboratory 12: | Preparation of DNA from and Agarose Gel | |
| Laboratory 13-14: | Labeling, Hybridization and Detection of DNA Probes | |
| Laboratory 15-21: | Subcloning into an expression vector | |
| Laboratory 21-23: | Preparation of Muscle Proteins | |
| Laboratory 22-23: | Expression and Purification of Protein | |
| Laboratory 23: | Polyacrylamide gel | |